siRNA knockdown validation 101: Incorporating negative controls in antibody research
نویسندگان
چکیده
More antibody validation protocols would identify non-specific reagents - a major source of irreproducible research - with the inclusion of negative controls. This article presents an overview of one such method: siRNA knockdown. The authors outline a general protocol, the knockdown mechanism, and tips for evaluating knockdown experiments.
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عنوان ژورنال:
دوره 5 شماره
صفحات -
تاریخ انتشار 2016